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Precise determination of RNA-protein contact sites in the 50 S ribosomal subunit of Escherichia coli.


ABSTRACT: RNA-protein cross-linked complexes were isolated and purified to obtain precise data about RNA-protein contact sites in the 50 S ribosomal subunit of Escherichia coli. N-terminal microsequencing and matrix-assisted laser desorption ionization MS were used to identify the cross-linking sites at the amino acid and nucleotide levels. In this manner the following contact sites of five ribosomal proteins with the 23 S rRNA were established: Lys-67 of L2 to U-1963, Tyr-35 of L4 to U-615, Lys-97 of L21 to U-546, Lys-49 of L23 to U-139 or C-140 and Lys-71 and Lys-74 of L27 to U-2334.

SUBMITTER: Thiede B 

PROVIDER: S-EPMC1219658 | biostudies-other | 1998 Aug

REPOSITORIES: biostudies-other

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