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Occurrence and expression of members of the ferritin gene family in cowpeas.

ABSTRACT: Ferritin gene expression has been demonstrated in a variety of plants including maize, Arabidopsis, cowpeas, soybeans, beans and peas. Most available evidence shows that the mature protein is located in plastids and its production is under gene transcriptional control. In maize, two different ferritin genes have been identified; they were found to express protein under different physiological conditions. Only single gene products have been found until now in the other plants, with the exception of cowpeas (Vigna unguiculata). Our previous work with cowpeas [Wicks and Entsch (1993) Biochem. Biophys. Res. Commun. 192, 813-819] showed the existence of a family of at least three ferritin genes, each coding for a protein subunit with a unique amino acid sequence. Here we report the discovery of a fourth active gene in cowpeas and present the full cDNA sequences for two of the four known members of the cowpea gene family. We also provide preliminary evidence for a family of ferritin genes in soybeans (Glycine max) related to that in cowpeas. We conclude that a family of genes is probably present in all higher plants. We have used quantitative reverse transcriptase-mediated PCR to show that each of the four members of the cowpea ferritin gene family expresses mRNA in leaves and roots under normal growth with a complete nutrient supply. The results clearly show a marked differential pattern of mRNA levels formed during development from the four genes. We conclude that the composition of plant ferritin molecules from plant leaf extracts is probably a complex mixture of subunits, which might be different in roots and in leaves.

SUBMITTER: Wardrop AJ

PROVIDER: S-EPMC1220005 | biostudies-other | 1999 Feb

REPOSITORIES: biostudies-other

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Project description:The tumor necrosis factor α-induced protein 8 (TNFAIP8)-like (TIPE) protein family comprises four members, namely TNFAIP8, TIPE1, TIPE2 and TIPE3, which are involved in multiple processes in cancer. The current study aimed to investigate the expression patterns and potential clinical roles of the TIPE family members in human colorectal cancer (CRC). Paired tumor and adjacent tissue samples were collected from 49 patients with CRC, and the relative mRNA expression levels of the TIPE family members in these samples were evaluated by using reverse transcription-quantitative PCR, and the protein levels in five randomly selected pairs of tumor and adjacent tissue samples were detected by western blot analysis. The mRNA expression levels of the TIPE family members were significantly downregulated in CRC tumor tissues compared with those in the adjacent tissues; however, within each sample, TNFAIP8 and TIPE3 protein levels were only partially consistent with their mRNA levels. In addition, the mRNA expression levels between each pair of TIPE family members exhibited a positive linear relationship, and TIPE2 mRNA levels exhibited strong linear associations with those of TNFAIP8 and TIPE1. TNFAIP8 mRNA expression levels in tumor tissues were significantly associated with the tumor differentiation grade, and TIPE2 mRNA expression levels in tumor tissues were significantly associated with sex. TIPE1 and TIPE3 mRNA expression levels in tumor tissues exhibited no associations with patient clinicopathological characteristics. In addition, the mRNA expression patterns of the TIPE family members were analyzed using data from The Cancer Genome Atlas data set, and the results also demonstrated that TNFAIP8, TIPE2 and TIPE3 mRNA levels were downregulated in patients with colon adenocarcinoma compared with those in normal controls. These results provided evidence that the four members of the TIPE family may affect each other to mediate the carcinogenesis of CRC, and that TIPE2 may serve an important role in CRC.

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Occurrence and expression of members of the ferritin gene family in cowpeas.

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