Unknown

Dataset Information

0

Fibroblast growth factor-1 interacts with the glucose-regulated protein GRP75/mortalin.


ABSTRACT: Fibroblast growth factor-1 (FGF-1), which lacks a signal peptide and is intracellularly localized as a result of endogenous expression or endocytosis, is thought to be involved in regulating cell growth and differentiation. In the study reported here, we purified proteins that bind intracellular FGF-1. Affinity adsorption was used to purify FGF-1-binding proteins from rat L6 cells expressing FGF-1. One of the isolated proteins was identified as the glucose-regulated protein GRP75/mortalin/PBP-74/mthsp70, a member of the hsp70 family of heat-shock proteins known to be involved in regulating glucose responses, antigen processing and cell mortality. The interaction of FGF-1 and GRP75/mortalin in vivo was confirmed by co-immunoprecipitation, immunohistochemical co-localization in Rat-1 fibroblasts and by using the yeast two-hybrid system. Moreover, a binding assay in vitro with the use of recombinant FGF-1 and mortalin demonstrated a direct physical interaction between the two proteins. These results reveal that GRP75/mortalin is an intracellular FGF-1-binding protein in cells and suggest that GRP75/mortalin is involved in the trafficking of and/or signalling by FGF-1.

SUBMITTER: Mizukoshi E 

PROVIDER: S-EPMC1220575 | biostudies-other | 1999 Oct

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC6538939 | biostudies-literature
| S-EPMC87226 | biostudies-literature
| S-EPMC4264065 | biostudies-literature
| S-EPMC4786731 | biostudies-literature
| S-EPMC7465916 | biostudies-literature
| S-EPMC8196146 | biostudies-literature
| S-EPMC7290722 | biostudies-literature
| S-EPMC4697173 | biostudies-literature
| S-EPMC3603371 | biostudies-literature
| S-EPMC3436551 | biostudies-literature