Unknown

Dataset Information

0

Overexpression of matrix metalloproteinase-2 mediates phenotypic transformation of lens epithelial cells.


ABSTRACT: Transforming growth factor-beta (TGF-beta) is known to be a causative factor in pathological fibrosis and the metastasis of cancer cells, through effects on molecules of the extracellular matrix (ECM). We evaluated the influence of TGF-beta(1) on the gene expression of matrix metalloproteinase-2 (MMP-2) in lens epithelial cells (LECs). The results showed that TGF-beta(1) induced the expression of mRNA for MMP-2 in LECs. Subsequently, in order to examine the role of MMP-2, we overexpressed MMP-2 in LECs by stable transfection. The MMP-2-overexpressing LECs showed typical indicators of a myofibroblast-like cell phenotype, such as multiple layers of cells, elongated morphology, and expression of alpha-smooth muscle actin. We also showed that an MMP inhibitor blocked the TGF-beta(1)-induced morphological change in LECs. These results demonstrate that MMP-2 plays a role in the transformation of LECs, which has implications for the pathological fibrosis of these cells.

SUBMITTER: Seomun Y 

PROVIDER: S-EPMC1222030 | biostudies-other | 2001 Aug

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC489981 | biostudies-literature
| S-EPMC2832147 | biostudies-literature
| S-EPMC2773913 | biostudies-literature
| S-EPMC3260085 | biostudies-literature
| S-EPMC6831093 | biostudies-literature
| S-EPMC8345112 | biostudies-literature
| S-EPMC2753283 | biostudies-literature
| S-EPMC2716958 | biostudies-literature
2007-12-18 | GSE8328 | GEO
| S-EPMC2792911 | biostudies-literature