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Alpha-, beta- or gamma-chain-specific RNA interference of laminin assembly in Drosophila Kc167 cells.

ABSTRACT: Drosophila laminin alphabetagamma trimer assembly in Kc167 cells was perturbed by chain-specific RNA interference (RNAi). The intracellular pool of alpha and gamma chains remained unchanged under beta-chain RNAi by lipofection of double-stranded RNA encoding a beta-chain partial sequence. This was also the case for the intracellular pool of alpha and beta chains under gamma-chain-specific RNAi. Nonetheless, the intracellular pool of beta and gamma chains increased markedly under alpha-chain-specific RNAi. Non-reducing SDS/PAGE revealed that some of the increased beta and gamma chains migrated as disulphide-linked betagamma dimers but that the rest migrated as monomers. Since the monomeric beta and gamma bands detected under alpha-chain RNAi were denser than the beta band under gamma-chain RNAi and the gamma band under beta-chain RNAi, respectively, beta and gamma also appeared to accumulate by forming betagamma dimers without the disulphide linkage. We suggest that interconversion of these betagamma dimers is crucial for the replaceable and selective assembly of the alpha chain for alphabetagamma trimer formation.

SUBMITTER: Goto A

PROVIDER: S-EPMC1222214 | biostudies-other | 2001 Nov

REPOSITORIES: biostudies-other

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wing blister, a new Drosophila laminin alpha chain required for cell adhesion and migration during embryonic and imaginal development.

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2008-10-21 | E-GEOD-12049 | biostudies-arrayexpress

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Project description:Gamma chain microbiota

| PRJEB6359 | ENA

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Expression data from laminin alpha 2 chain deficient mice vs wild type

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