Unknown

Dataset Information

0

Post-synaptic density-95 promotes calcium/calmodulin-dependent protein kinase II-mediated Ser847 phosphorylation of neuronal nitric oxide synthase.


ABSTRACT: Post-synaptic density-95 (PSD-95) is a neuronal scaffolding protein that associates with N -methyl-D-aspartate (NMDA) receptors and links them to intracellular signalling molecules. In neurons, neuronal nitric oxide synthase (nNOS) binds selectively to the second PDZ domain (PDZ2) of PSD-95, thereby exhibiting physiological activation triggered via NMDA receptors. We have demonstrated previously that Ca(2+)/calmodulin-dependent protein kinase IIalpha (CaM-K IIalpha) directly phosphorylates nNOS at residue Ser(847), and can attenuate the catalytic activity of the enzyme in neuronal cells [Komeima, Hayashi, Naito and Watanabe (2000) J. Biol. Chem. 275, 28139-28143]. In the present study, we examined how CaM-K II participates in the phosphorylation by analysing the functional interaction between nNOS and PSD-95 in cells. The results showed that PSD-95 directly promotes the nNOS phosphorylation at Ser(847) induced by endogenous CaM-K II. In transfected cells, this effect of PSD-95 required its dual palmitoylation and the PDZ2 domain, but did not rely on its guanylate kinase domain. CaM-K Ialpha and CaM-K IV failed to phosphorylate nNOS at Ser(847) in transfected cells. Thus PSD-95 mediates cellular trafficking of nNOS, and may be required for the efficient phosphorylation of nNOS at Ser(847) by CaM-K II in neuronal cells.

SUBMITTER: Watanabe Y 

PROVIDER: S-EPMC1223425 | biostudies-other | 2003 Jun

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC3387242 | biostudies-literature
| S-EPMC2198825 | biostudies-literature
| S-EPMC7039552 | biostudies-literature
| S-EPMC2758698 | biostudies-literature
| S-EPMC3569036 | biostudies-literature
| S-EPMC6303682 | biostudies-literature
| S-EPMC3281602 | biostudies-literature
| S-EPMC1618497 | biostudies-literature
| S-EPMC145438 | biostudies-literature
| S-EPMC337024 | biostudies-literature