Simple, robust methods for high-throughput nanoliter-scale DNA sequencing.
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ABSTRACT:
SUBMITTER: Smailus DE
PROVIDER: S-EPMC1240088 | biostudies-other | 2005 Oct
REPOSITORIES: biostudies-other
Publications
Simple, robust methods for high-throughput nanoliter-scale DNA sequencing.
Smailus Duane E DE Marziali Andre A Dextras Philip P Marra Marco A MA Holt Robert A RA
Genome research 20050916 10
We have developed high-throughput DNA sequencing methods that generate high quality data from reactions as small as 400 nL, providing an approximate order of magnitude reduction in reagent use relative to standard protocols. Sequencing of clones from plasmid, fosmid, and BAC libraries yielded read lengths (PHRED20 bases) of 765 +/- 172 (n = 10,272), 621 +/- 201 (n = 1824), and 647 +/- 189 (n = 568), respectively. Implementation of these procedures at high-throughput genome centers could have a s ...[more]
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