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Purification of beta-acetylglucosaminase and beta-galactosidase from ram testis.

ABSTRACT: 1. The presence of beta-galactosidase (EC 3.2.1.23) in an acetic acid extract of ram testis is reported. Some properties of the crude enzyme preparation were studied. 2. The purification of beta-acetylglucosaminase (EC 3.2.1.30) and of beta-galactosidase from the ram-testis extract by ammonium sulphate precipitation and chromatography on a CM-cellulose column is described. 3. The final purifications of the separated enzymes achieved were for the beta-acetylglucosaminase 35 times and for the beta-galactosidase 99 times. 4. The possibility of using DEAE-cellulose and Sephadex G-200 to purify the enzymes was investigated.

SUBMITTER: Caygill JC 

PROVIDER: S-EPMC1264859 | biostudies-other | 1966 Feb

REPOSITORIES: biostudies-other

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