Unknown

Dataset Information

0

Effect of cycloheximide on protein and ribonucleic acid synthesis in cultured human lymphocytes.


ABSTRACT: 1. Phytohaemagglutinin stimulates the transformation into blast cells of human lymphocytes incubated in vitro. This transformation is accompanied by an increase in the incorporation of [(14)C]leucine into protein and [(3)H]uridine into RNA. 2. The incorporation of [(14)C]leucine by cultures grown in the presence or absence of phytohaemagglutinin is inhibited to the same extent by cycloheximide, a known inhibitor of protein synthesis. 3. Lymphocytes grown without phytohaemagglutin synthesize mainly non-ribosomal RNA. [(3)H]Uridine incorporation by these cells was increased by cycloheximide. 4. Lymphocytes incubated with phytohaemagglutinin begin to synthesize substantial quantities of ribosomal RNA. Under these conditions [(3)H]uridine incorporation was partially inhibited by cycloheximide. This inhibition is shown to be largely a result of inhibition of the synthesis of ribosomal RNA.

SUBMITTER: Kay JE 

PROVIDER: S-EPMC1265220 | biostudies-other | 1966 Sep

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1184117 | biostudies-other
| S-EPMC1166029 | biostudies-other
| S-EPMC8663959 | biostudies-literature
| S-EPMC1161736 | biostudies-other
| S-EPMC5928012 | biostudies-literature
| S-EPMC2782634 | biostudies-literature
| S-EPMC7059790 | biostudies-literature
| S-EPMC1177579 | biostudies-other
| S-EPMC1165390 | biostudies-other
| S-EPMC1165748 | biostudies-other