Acid mucopolysaccharides of chick-embryo cartilage in osteolathyrism.
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ABSTRACT:
SUBMITTER: Levene CI
PROVIDER: S-EPMC1270125 | biostudies-other | 1966 Nov
REPOSITORIES: biostudies-other
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Project description:1. Treatment of the chick embryo with the lathyrus factor, beta-aminopropionitrile, produces inhibition of polymerization of newly synthesized collagen at the interand intra-molecular level (Levene & Gross, 1959; Martin, Gross, Piez & Lewis, 1961); this is manifest as an increase in the fragility and solubility of collagen from the connective tissues. 2. This treatment simultaneously produced increased hydration of the tissues, particularly noticeable in the swollen long-bone cartilages. 3. This increased hydration of cartilage was found to result from treatment with any of the known lathyrogenic compounds, but not with any of the structurally related but non-lathyrogenic analogues. 4. The hydration effect paralleled the collagen solubility effect, both being dosage-dependent at the lower levels. 5. The hydration effect could not be reversed by treatment in vivo with either pyridoxal or glyceraldehyde, or by Benadryl, an antihistamine that inhibits cell water movement. 6. The possible causes of this effect have been considered in the light of the known properties of cartilage.
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Project description:BackgroundLittle is known regarding the molecular pathways that underlie the process of retinal development. The purpose of this study was to identify proteins which may be involved in development of retina. We used a proteomics-based approach to identify proteins that are up- or down-regulated during the development of the embryo chick retina.ResultsTwo-dimensional gel electrophoresis was performed with the retina of embryo chicken, which was obtained from embryos of day 7 (ED7) and of day 11 (ED11). The protein spots showing significant differences were selected for identification by MALDI mass spectrometry. Thirteen proteins were differentially expressed; seven proteins were up-regulated in embryo retina of chicken at ED 11 and six proteins were down-regulated. Significant proteins were also evaluated in embryo day 15 (ED15). Some of identified proteins were known to regulate cell proliferation, cell death, transport, metabolism, organization and extracellular matrix, and others also included novel proteins.ConclusionWe identified thirteen proteins which differentially expressed in embryonal retina of chicken at day 7, as compared to the retina of embryo of day 11. They were various regulatory proteins for cellular signaling.
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