Unknown

Dataset Information

0

The metabolism of phospholipids in mouse brain slices.


ABSTRACT: 1. Slices of mouse brain grey matter were incubated with [(32)P]phosphate and [1-(14)C]acetate. Doubly labelled phospholipids were extracted from subcellular fractions prepared from the slices in a mixture of metabolic inhibitors, under conditions where there was negligible change in radioactive labelling during the preparation. Two tissue fractions were studied in detail; one contained a high proportion of mitochondria and the other was mainly microsomal. 2. In all tissue fractions the highest incorporations of both [(32)P]phosphate and [1-(14)C]acetate occurred into phosphatidylcholine. 3. After incubation for 1hr., the (32)P/(14)C ratios for phosphatidylcholine, phosphatidylethanolamine and phosphatidic acid in the mitochondrial fraction were similar to those in the microsomal fraction. 4. The (32)P/(14)C ratios were similar in phosphatidylcholine and phosphatidylethanolamine and much lower than those in phosphatidic acid and phosphatidylinositol.

SUBMITTER: Clayton PA 

PROVIDER: S-EPMC1270171 | biostudies-other | 1966 Dec

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC8082162 | biostudies-literature
| S-EPMC6981710 | biostudies-literature
| S-EPMC1179565 | biostudies-other
| S-EPMC2817596 | biostudies-literature
| S-EPMC6139133 | biostudies-literature
| S-EPMC3906077 | biostudies-literature
| S-EPMC7359299 | biostudies-literature
| S-EPMC10661668 | biostudies-literature
| S-EPMC5969222 | biostudies-literature
| S-EPMC5882520 | biostudies-literature