Ontology highlight
ABSTRACT:
SUBMITTER: Schwaiger I
PROVIDER: S-EPMC1299227 | biostudies-other | 2005 Jan
REPOSITORIES: biostudies-other
Schwaiger Ingo I Schleicher Michael M Noegel Angelika A AA Rief Matthias M
EMBO reports 20050101 1
The F-actin crosslinker filamin from Dictyostelium discoideum (ddFLN) has a rod domain consisting of six structurally similar immunoglobulin domains. When subjected to a stretching force, domain 4 unfolds at a lower force than all the other domains in the chain. Moreover, this domain shows a stable intermediate along its mechanical unfolding pathway. We have developed a mechanical single-molecule analogue to a double-jump stopped-flow experiment to investigate the folding kinetics and pathway of ...[more]