Ontology highlight
ABSTRACT:
SUBMITTER: Fitter J
PROVIDER: S-EPMC1301055 | biostudies-other | 2000 Sep
REPOSITORIES: biostudies-other
Biophysical journal 20000901 3
By comparing a mesophilic alpha-amylase with its thermophilic homolog, we investigated the relationship between thermal stability and internal equilibrium fluctuations. Fourier transform infrared spectroscopy monitoring hydrogen/deuterium (H/D) exchange kinetics and incoherent neutron scattering measuring picosecond dynamics were used to study dynamic features of the folded state at room temperature. Fairly similar rates of slowly exchanging amide protons indicate about the same free energy of s ...[more]