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Fabrication of nanometer-sized protein patterns using atomic force microscopy and selective immobilization.

ABSTRACT: A new methodology is introduced to produce nanometer-sized protein patterns. The approach includes two main steps, nanopatterning of self-assembled monolayers using atomic force microscopy (AFM)-based nanolithography and subsequent selective immobilization of proteins on the patterned monolayers. The resulting templates and protein patterns are characterized in situ using AFM. Compared with conventional protein fabrication methods, this approach is able to produce smaller patterns with higher spatial precision. In addition, fabrication and characterization are completed in near physiological conditions. The adsorption configuration and bioreactivity of the proteins within the nanopatterns are also studied in situ.

SUBMITTER: Wadu-Mesthrige K

PROVIDER: S-EPMC1301377 | biostudies-other | 2001 Apr

REPOSITORIES: biostudies-other

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Fabrication of nanometer-sized protein patterns using atomic force microscopy and selective immobilization.

Wadu-Mesthrige K K Amro N A NA Garno J C JC Xu S S Liu G G

Biophysical journal 20010401 4

A new methodology is introduced to produce nanometer-sized protein patterns. The approach includes two main steps, nanopatterning of self-assembled monolayers using atomic force microscopy (AFM)-based nanolithography and subsequent selective immobilization of proteins on the patterned monolayers. The resulting templates and protein patterns are characterized in situ using AFM. Compared with conventional protein fabrication methods, this approach is able to produce smaller patterns with higher sp ...[more]

PMID: 11259301

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