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Diffusion-collision model study of misfolding in a four-helix bundle protein.

ABSTRACT: Proteins with complex folding kinetics will be susceptible to misfolding at some stage in the folding process. We simulate this problem by using the diffusion-collision model to study non-native kinetic intermediate misfolding in a four-helix bundle protein. We find a limit on the size of the pairwise hydrophobic area loss in non-native intermediates, such that burying above this limit creates long-lasting non-native kinetic intermediates that would disrupt folding and prevent formation of the native state. Our study of misfolding suggests a method for limiting the production of misfolded kinetic intermediates for helical proteins and could, perhaps, lead to more efficient production of proteins in bulk.

SUBMITTER: Beck C

PROVIDER: S-EPMC1301772 | biostudies-other | 2001 Dec

REPOSITORIES: biostudies-other

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Diffusion-collision model study of misfolding in a four-helix bundle protein.

Beck C C Siemens X X Weaver D L DL

Biophysical journal 20011201 6

Proteins with complex folding kinetics will be susceptible to misfolding at some stage in the folding process. We simulate this problem by using the diffusion-collision model to study non-native kinetic intermediate misfolding in a four-helix bundle protein. We find a limit on the size of the pairwise hydrophobic area loss in non-native intermediates, such that burying above this limit creates long-lasting non-native kinetic intermediates that would disrupt folding and prevent formation of the n ...[more]

PMID: 11720978

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