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Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins.

ABSTRACT: We have identified an apparent human homolog of the yeast Nop5/Nop58 protein. hNop5/Nop58 codes for a protein of predicted molecular weight 59.6 kDa and is 46.8% identical to Saccharomyces cerevisiae Nop5/Nop58. Immunofluorescent staining with antibodies against hNop5/Nop58 indicate that it is localized primarily to the nucleolus, and coimmunoprecipitation from nuclear extracts demonstrates that hNop5/Nop58 interacts with the box C/D family of snoRNAs. Thus, hNop5/Nop58 is a common component of the box C/D snoRNPs, and joins fibrillarin as the second such component identified and characterized in metazoans.

SUBMITTER: Lyman SK

PROVIDER: S-EPMC1369881 | biostudies-other | 1999 Dec

REPOSITORIES: biostudies-other

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Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins.

Lyman S K SK Gerace L L Baserga S J SJ

RNA (New York, N.Y.) 19991201 12

We have identified an apparent human homolog of the yeast Nop5/Nop58 protein. hNop5/Nop58 codes for a protein of predicted molecular weight 59.6 kDa and is 46.8% identical to Saccharomyces cerevisiae Nop5/Nop58. Immunofluorescent staining with antibodies against hNop5/Nop58 indicate that it is localized primarily to the nucleolus, and coimmunoprecipitation from nuclear extracts demonstrates that hNop5/Nop58 interacts with the box C/D family of snoRNAs. Thus, hNop5/Nop58 is a common component of ...[more]

PMID: 10606270

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