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Identification and punctate nuclear localization of a novel noncoding RNA, Ks-1, from the honeybee brain.

ABSTRACT: We identified a novel gene, Ks-1, which is expressed preferentially in the small-type Kenyon cells of the honeybee brain. This gene is also expressed in some of the large soma neurons in the brain and in the suboesophageal ganglion. Reverse transcription-polymerase chain reaction experiments indicated that Ks-1 transcripts are enriched in the honeybee brain. cDNA cloning revealed that the consensus Ks-1 cDNA is over 17 kbp and contains no significant open reading frames. Furthermore, fluorescent in situ hybridization revealed that Ks-1 transcripts are located in the nuclei of the neural cells, accumulating in some scattered spots. These findings demonstrate that Ks-1 encodes a novel class of noncoding nuclear RNA and is possibly involved in the regulation of neural functions.

SUBMITTER: Sawata M

PROVIDER: S-EPMC1370296 | biostudies-other | 2002 Jun

REPOSITORIES: biostudies-other

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Identification and punctate nuclear localization of a novel noncoding RNA, Ks-1, from the honeybee brain.

Sawata Miyuki M Yoshino Daisuke D Takeuchi Hideaki H Kamikouchi Azusa A Ohashi Kazuaki K Kubo Takeo T

RNA (New York, N.Y.) 20020601 6

We identified a novel gene, Ks-1, which is expressed preferentially in the small-type Kenyon cells of the honeybee brain. This gene is also expressed in some of the large soma neurons in the brain and in the suboesophageal ganglion. Reverse transcription-polymerase chain reaction experiments indicated that Ks-1 transcripts are enriched in the honeybee brain. cDNA cloning revealed that the consensus Ks-1 cDNA is over 17 kbp and contains no significant open reading frames. Furthermore, fluorescent ...[more]

PMID: 12088150

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