Improvement of oligonucleotide probe design criteria for functional gene microarrays in environmental applications.
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ABSTRACT:
SUBMITTER: Liebich J
PROVIDER: S-EPMC1392891 | biostudies-other | 2006 Feb
REPOSITORIES: biostudies-other
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Improvement of oligonucleotide probe design criteria for functional gene microarrays in environmental applications.
Liebich Jost J Schadt Christopher W CW Chong Song C SC He Zhili Z Rhee Sung-Keun SK Zhou Jizhong J
Applied and environmental microbiology 20060201 2
To optimize oligonucleotide probe design criteria, PCR products with different similarities to probes were hybridized to a functional gene microarray designed to detect homologous genes from different organisms. In contrast to more restrictive probe designs based on a single criterion, simultaneous consideration of the percent similarity (< or =90%), the length of identical sequence stretches (< or =20 bases), and the binding free energy (> or =-35 kcal mol(-1)) was found to be predictive of pro ...[more]
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