Ontology highlight
ABSTRACT:
SUBMITTER: Kim EY
PROVIDER: S-EPMC1440878 | biostudies-other | 2006 Apr
REPOSITORIES: biostudies-other
Kim Eun-Young EY Stanton Jennifer J Vega Rafael A RA Kunstman Kevin J KJ Mirkin Chad A CA Wolinsky Steven M SM
Nucleic acids research 20060414 7
Here we report a real-time PCR-based method for determining the surface coverage of dithiol-capped oligonucleotides bound onto gold nanoparticles alone and in tandem with antibody. The detection of gold nanoparticle-bound DNA is accomplished by targeting the oligonucleotide with primer and probe binding sites, amplification of the oligonucleotide by PCR, and real-time measurement of the fluorescence emitted during the reaction. This method offers a wide dynamic range and is not dependant on the ...[more]