Ontology highlight
ABSTRACT:
SUBMITTER: Vidali L
PROVIDER: S-EPMC1446085 | biostudies-other | 2006 May
REPOSITORIES: biostudies-other
Vidali Luis L Chen Feng F Cicchetti Gregor G Ohta Yasutaka Y Kwiatkowski David J DJ
Molecular biology of the cell 20060308 5
Previous studies of Rac1 in fibroblasts have used dominant negative constructs, which may have nonspecific effects. We used a conditional Rac1 allele to critically examine Rac1 function in mouse fibroblasts. Lack of Rac1 had dramatic effects on nonconfluent cells, which were elongated and had extensive blebbing, but no lamellipodia or ruffle formation. However, Rac1-null fibroblasts translocated using pseudopodia-like protrusions without lamellipodia, migrating toward a platelet-derived growth f ...[more]