Unknown

Dataset Information

0

TRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.


ABSTRACT: We describe a program, tRNAscan-SE, which identifies 99-100% of transfer RNA genes in DNA sequence while giving less than one false positive per 15 gigabases. Two previously described tRNA detection programs are used as fast, first-pass prefilters to identify candidate tRNAs, which are then analyzed by a highly selective tRNA covariance model. This work represents a practical application of RNA covariance models, which are general, probabilistic secondary structure profiles based on stochastic context-free grammars. tRNAscan-SE searches at approximately 30 000 bp/s. Additional extensions to tRNAscan-SE detect unusual tRNA homologues such as selenocysteine tRNAs, tRNA-derived repetitive elements and tRNA pseudogenes.

SUBMITTER: Lowe TM 

PROVIDER: S-EPMC146525 | biostudies-other | 1997 Mar

REPOSITORIES: biostudies-other

altmetric image

Publications

tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.

Lowe T M TM   Eddy S R SR  

Nucleic acids research 19970301 5


We describe a program, tRNAscan-SE, which identifies 99-100% of transfer RNA genes in DNA sequence while giving less than one false positive per 15 gigabases. Two previously described tRNA detection programs are used as fast, first-pass prefilters to identify candidate tRNAs, which are then analyzed by a highly selective tRNA covariance model. This work represents a practical application of RNA covariance models, which are general, probabilistic secondary structure profiles based on stochastic c  ...[more]

Similar Datasets

| S-EPMC8450103 | biostudies-literature
| S-EPMC2695483 | biostudies-literature
| S-EPMC1152862 | biostudies-other
| S-EPMC2475316 | biostudies-other
| S-EPMC3280502 | biostudies-literature
| S-EPMC310774 | biostudies-literature
| S-EPMC6130373 | biostudies-other
| S-EPMC3315322 | biostudies-literature
2014-04-25 | E-GEOD-54333 | biostudies-arrayexpress
| S-EPMC318170 | biostudies-other