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TRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.


ABSTRACT: We describe a program, tRNAscan-SE, which identifies 99-100% of transfer RNA genes in DNA sequence while giving less than one false positive per 15 gigabases. Two previously described tRNA detection programs are used as fast, first-pass prefilters to identify candidate tRNAs, which are then analyzed by a highly selective tRNA covariance model. This work represents a practical application of RNA covariance models, which are general, probabilistic secondary structure profiles based on stochastic context-free grammars. tRNAscan-SE searches at approximately 30 000 bp/s. Additional extensions to tRNAscan-SE detect unusual tRNA homologues such as selenocysteine tRNAs, tRNA-derived repetitive elements and tRNA pseudogenes.

SUBMITTER: Lowe TM 

PROVIDER: S-EPMC146525 | biostudies-other | 1997 Mar

REPOSITORIES: biostudies-other

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tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.

Lowe T M TM   Eddy S R SR  

Nucleic acids research 19970301 5


We describe a program, tRNAscan-SE, which identifies 99-100% of transfer RNA genes in DNA sequence while giving less than one false positive per 15 gigabases. Two previously described tRNA detection programs are used as fast, first-pass prefilters to identify candidate tRNAs, which are then analyzed by a highly selective tRNA covariance model. This work represents a practical application of RNA covariance models, which are general, probabilistic secondary structure profiles based on stochastic c  ...[more]

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