Ontology highlight
ABSTRACT:
SUBMITTER: Valk PJ
PROVIDER: S-EPMC147027 | biostudies-other | 1997 Nov
REPOSITORIES: biostudies-other
Valk P J PJ Joosten M M Vankan Y Y Löwenberg B B Delwel R R
Nucleic acids research 19971101 21
Proto-oncogenes in retrovirally induced myeloid mouse leukemias are frequently activated following retroviral insertion. The identification of common virus integration sites (VISs) and isolation of the transforming oncogene is laborious and time consuming. We established a rapid and simple PCR based procedure which facilitates the identification of VISs and novel proto-oncogenes. Complementary DNA fragments adjacent to retrovirus integration sites were selectively isolated by applying a reverse ...[more]