A novel quantitative multiplex NASBA method: application to measuring tissue factor and CD14 mRNA levels in human monocytes.
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ABSTRACT:
SUBMITTER: van Deursen PB
PROVIDER: S-EPMC148576 | biostudies-other | 1999 Sep
REPOSITORIES: biostudies-other
Publications
A novel quantitative multiplex NASBA method: application to measuring tissue factor and CD14 mRNA levels in human monocytes.
van Deursen P B PB Gunther A W AW van Riel C C CC van der Eijnden M M MM Vos H L HL van Gemen B B van Strijp D A DA Tackent N M NM Bertina R M RM
Nucleic acids research 19990901 17
A new method to quantify two individual mRNAs in a single NASBA reaction is described. In this study, tissue factor and CD14 mRNAs were used as a model system. RNA ratios of -4 to +4 log units were determined with good precision (within 0.3 log) and accuracy (within 0.2 log). By measuring both mRNAs in human monocytes that were stimulated with LPS, the multiplex Q-NASBA proved to be a successful tool to monitor the expression levels of two individual mRNAs in a single-tube amplification system. ...[more]
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