Ontology highlight
ABSTRACT:
SUBMITTER: Wohlgemuth I
PROVIDER: S-EPMC1500836 | biostudies-other | 2006 Jul
REPOSITORIES: biostudies-other
Wohlgemuth Ingo I Beringer Malte M Rodnina Marina V MV
EMBO reports 20060616 7
The catalytic site of the ribosome, the peptidyl transferase centre, is located on the large (50S in bacteria) ribosomal subunit. On the basis of results obtained with small substrate analogues, isolated 50S subunits seem to be less active in peptide bond formation than 70S ribosomes by several orders of magnitude, suggesting that the reaction mechanisms on 50S subunits and 70S ribosomes may be different. Here we show that with full-size fMet-tRNA(fMet) and puromycin or C-puromycin as peptide do ...[more]