Unknown

Dataset Information

0

Pharmacological comparison of the alternatively spliced short and long CCK2 receptors.


ABSTRACT: (1) The alternatively spliced, short and long cholecystokinin receptors (CCK2S and CCK2L) were expressed in NIH3T3 cells, and compared using radioligand-binding assays with identical buffer and incubation conditions. (2) As judged by a saturation analysis, the selective CCK2-receptor antagonist radioligand [3H]-JB93182 did not discriminate between the CCK2S or CCK2L receptors. (3) A global analysis of competition studies, using a range of structurally diverse, CCK-receptor selective ligands, provided further evidence that these receptor subtypes were pharmacologically indistinguishable. However, when analysed individually a number of small, yet significant differences were observed with some of the compounds. (4) These data are consistent with previous study that suggested a possible pharmacological difference between these isoforms, at least in terms of the CCK2-receptor antagonist, L-365,260. However, it would appear that the pharmacological profile of these compounds is not consistent with their affinity at the putative G1/G2 receptors previously described by Harper et al.

SUBMITTER: Morton MF 

PROVIDER: S-EPMC1574017 | biostudies-other | 2003 Sep

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC6740967 | biostudies-literature
| S-EPMC6880636 | biostudies-literature
| S-EPMC21274 | biostudies-literature
| S-EPMC308870 | biostudies-literature
| S-EPMC186627 | biostudies-literature
| S-EPMC102426 | biostudies-literature
| S-EPMC1616956 | biostudies-literature
| S-EPMC1185642 | biostudies-literature
| S-EPMC2839007 | biostudies-literature
| S-EPMC2651755 | biostudies-literature