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A novel integron-like element carrying the metallo-beta-lactamase gene blaIMP.

ABSTRACT: A plasmid-mediated metallo-beta-lactamase gene was cloned from a carbapenem-resistant Serratia marcescens strain, AK9373. The metallo-beta-lactamase gene was identical to the blaIMP, and it was located in the space between an integrase-like gene and an aac(6')-Ib-like gene. The deduced amino acid sequence for the putative integrase gene showed considerable identity (60.9%) to that of the Escherichia coli integrase reported. Sequences similar to the GTTRRRY and an atypical 59-base element containing a 67-bp inverted repeat sequence, which were peculiar to the integrase-dependent recombination, were also conserved in the flanking regions of the blaIMP gene. These findings imply that the metallo-beta-lactamase gene in S. marcescens AK9373 is carried by a novel integron-like element that is mediated by a transferable large plasmid.

SUBMITTER: Arakawa Y

PROVIDER: S-EPMC162793 | biostudies-other | 1995 Jul

REPOSITORIES: biostudies-other

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A novel integron-like element carrying the metallo-beta-lactamase gene blaIMP.

Arakawa Y Y Murakami M M Suzuki K K Ito H H Wacharotayankun R R Ohsuka S S Kato N N Ohta M M

Antimicrobial agents and chemotherapy 19950701 7

A plasmid-mediated metallo-beta-lactamase gene was cloned from a carbapenem-resistant Serratia marcescens strain, AK9373. The metallo-beta-lactamase gene was identical to the blaIMP, and it was located in the space between an integrase-like gene and an aac(6')-Ib-like gene. The deduced amino acid sequence for the putative integrase gene showed considerable identity (60.9%) to that of the Escherichia coli integrase reported. Sequences similar to the GTTRRRY and an atypical 59-base element contain ...[more]

PMID: 7492116

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