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Rapid detection of mutations associated with macrolide resistance in Mycobacterium avium complex.

ABSTRACT: Macrolide resistance in Mycobacterium avium can be detected with an adaption of a commercially available RNA/RNA duplex mismatch assay (Ambion, Austin, Tex.). The sensitivity and specificity values for the assay were 100% when evaluated against 41 macrolide-resistant and -susceptible strains of M. avium. Resistant subpopulations of approximately 20% could be readily detected. The assay is simple to perform and interpret, inexpensive, and rapid (< 24-h turnaround).

SUBMITTER: Nash KA

PROVIDER: S-EPMC163411 | biostudies-other | 1996 Jul

REPOSITORIES: biostudies-other

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Rapid detection of mutations associated with macrolide resistance in Mycobacterium avium complex.

Nash K A KA Inderlied C B CB

Antimicrobial agents and chemotherapy 19960701 7

Macrolide resistance in Mycobacterium avium can be detected with an adaption of a commercially available RNA/RNA duplex mismatch assay (Ambion, Austin, Tex.). The sensitivity and specificity values for the assay were 100% when evaluated against 41 macrolide-resistant and -susceptible strains of M. avium. Resistant subpopulations of approximately 20% could be readily detected. The assay is simple to perform and interpret, inexpensive, and rapid (< 24-h turnaround). ...[more]

PMID: 8807078

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