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Cloning and sequence analysis of a class A beta-lactamase from Mycobacterium tuberculosis H37Ra.


ABSTRACT: A cosmid library from Mycobacterium tuberculosis H37Ra was introduced into Mycobacterium smegmatis, and eight recombinant clones with increased resistance to cefoxitin were identified. Isoelectric focusing detected an M. tuberculosis-derived beta-lactamase in one of these recombinant clones. A sequence analysis identified it as a class A beta-lactamase whose expression correlated with the increased resistance phenotype.

SUBMITTER: Hackbarth CJ 

PROVIDER: S-EPMC163878 | biostudies-other | 1997 May

REPOSITORIES: biostudies-other

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Cloning and sequence analysis of a class A beta-lactamase from Mycobacterium tuberculosis H37Ra.

Hackbarth C J CJ   Unsal I I   Chambers H F HF  

Antimicrobial agents and chemotherapy 19970501 5


A cosmid library from Mycobacterium tuberculosis H37Ra was introduced into Mycobacterium smegmatis, and eight recombinant clones with increased resistance to cefoxitin were identified. Isoelectric focusing detected an M. tuberculosis-derived beta-lactamase in one of these recombinant clones. A sequence analysis identified it as a class A beta-lactamase whose expression correlated with the increased resistance phenotype. ...[more]

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