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Isolation of a gene encoding a novel spectinomycin phosphotransferase from Legionella pneumophila.


ABSTRACT: A gene capable of conferring spectinomycin resistance was isolated from Legionella pneumophila, the agent of Legionnaires' disease. The gene (aph) encoded a 36-kDa protein which has similarity to aminoglycoside phosphotransferases. Biochemical analysis confirmed that aph encodes a phosphotransferase which modifies spectinomycin but not hygromycin, kanamycin, or streptomycin. The strain that was the source of aph demonstrated resistance to spectinomycin, and Southern hybridizations determined that aph also exists in other legionellae.

SUBMITTER: Suter TM 

PROVIDER: S-EPMC163921 | biostudies-other | 1997 Jun

REPOSITORIES: biostudies-other

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Isolation of a gene encoding a novel spectinomycin phosphotransferase from Legionella pneumophila.

Suter T M TM   Viswanathan V K VK   Cianciotto N P NP  

Antimicrobial agents and chemotherapy 19970601 6


A gene capable of conferring spectinomycin resistance was isolated from Legionella pneumophila, the agent of Legionnaires' disease. The gene (aph) encoded a 36-kDa protein which has similarity to aminoglycoside phosphotransferases. Biochemical analysis confirmed that aph encodes a phosphotransferase which modifies spectinomycin but not hygromycin, kanamycin, or streptomycin. The strain that was the source of aph demonstrated resistance to spectinomycin, and Southern hybridizations determined tha  ...[more]

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