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An origin of DNA replication from Lactococcus lactis bacteriophage c2.


ABSTRACT: An origin of DNA relication was identified in the intergenic region between the early and late gene regions of prolate lactococcal phage c2. A DNA fragment containing this origin, designated ori, was shown to direct DNA replication in Lactococcus lactis but not in Escherichia coli. A comparison of ori with the corresponding regions of other prolate phages revealed strict conservation of the nucleotide sequence in one half of this intergenic region. This conserved region alone would not support DNA replication. No open reading frames were identified in the ori fragment, suggesting that host factors alone are sufficient to initiate DNA replication at ori. A novel class of lactococcal vectors and E. coli-L. lactis shuttle vectors based on ori have been constructed.

SUBMITTER: Waterfield NR 

PROVIDER: S-EPMC167916 | biostudies-other | 1996 Apr

REPOSITORIES: biostudies-other

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An origin of DNA replication from Lactococcus lactis bacteriophage c2.

Waterfield N R NR   Lubbers M W MW   Polzin K M KM   Le Page R W RW   Jarvis A W AW  

Applied and environmental microbiology 19960401 4


An origin of DNA relication was identified in the intergenic region between the early and late gene regions of prolate lactococcal phage c2. A DNA fragment containing this origin, designated ori, was shown to direct DNA replication in Lactococcus lactis but not in Escherichia coli. A comparison of ori with the corresponding regions of other prolate phages revealed strict conservation of the nucleotide sequence in one half of this intergenic region. This conserved region alone would not support D  ...[more]

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