Ontology highlight
ABSTRACT:
SUBMITTER: Rijpens NP
PROVIDER: S-EPMC167942 | biostudies-other | 1996 May
REPOSITORIES: biostudies-other
Rijpens N P NP Jannes G G Van Asbroeck M M Rossau R R Herman L M LM
Applied and environmental microbiology 19960501 5
The 16S-23S rRNA spacer regions of Brucella abortus, B. melitensis, and B. suis were cloned and subcloned after PCR amplification. Sequence analysis of the inserts revealed a spacer of about 800 bp with very high ( > 99%) homology among the three species examined. Two genus-specific primer pairs, BRU-P5-BRU-P8 and BRU-P6-BRU-P7, that could be used in a nested PCR format and three genus-specific DNA probes, BRU-ICG2, BRU-ICG3, and BRU-ICG4, were deduced from this spacer. The specificity and sensi ...[more]