Ontology highlight
ABSTRACT:
SUBMITTER: Adul Rahman RN
PROVIDER: S-EPMC168544 | biostudies-other | 1997 Jun
REPOSITORIES: biostudies-other
Applied and environmental microbiology 19970601 6
The glnA gene encoding glutamine synthetase was cloned from the hyperthermophilic archaeon Pyrococcus sp. strain KOD1, and its nucleotide sequence was determined. The glnA gene was expressed in Escherichia coli ME8459 (glnA mutant strain), and the protein was purified to homogeneity and shown to be functional in a dodecameric from (637,000 Da), exhibiting both transferase and synthetase activities. However, kinetic studies indicated that the enzyme possessed low biosynthetic activity, suggesting ...[more]