PCR-mediated detection of acidophilic, bioleaching-associated bacteria.
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ABSTRACT:
SUBMITTER: De Wulf-Durand P
PROVIDER: S-EPMC168590 | biostudies-other | 1997 Jul
REPOSITORIES: biostudies-other
Publications
PCR-mediated detection of acidophilic, bioleaching-associated bacteria.
De Wulf-Durand P P Bryant L J LJ Sly L I LI
Applied and environmental microbiology 19970701 7
The detection of acidophilic microorganisms from mining environments by culture methods is time consuming and unreliable. Several PCR approaches were developed to amplify small-subunit rRNA sequences from the DNA of six bacterial phylotypes associated with acidic mining environments, permitting the detection of the target DNA at concentrations as low as 10 fg. ...[more]
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