Ontology highlight
ABSTRACT:
SUBMITTER: Clayton MA
PROVIDER: S-EPMC173366 | biostudies-other | 1995 Jul
REPOSITORIES: biostudies-other
Clayton M A MA Clayton J M JM Brown D R DR Middlebrook J L JL
Infection and immunity 19950701 7
A completely synthetic gene encoding fragment C, a approximately 50-kDa fragment, of botulinum neurotoxin serotype A was constructed from oligonucleotides. The gene was expressed in Escherichia coli, and full-sized product was produced as judged by Western blot (immunoblot) analysis. Crude extracts of E. coli expressing the gene were used to vaccinate mice and evaluate their survival against challenge with active toxin. Mice given three subcutaneous vaccinations were protected against an intrape ...[more]