Ontology highlight
ABSTRACT:
SUBMITTER: Drazek ES
PROVIDER: S-EPMC173454 | biostudies-other | 1995 Sep
REPOSITORIES: biostudies-other
Drazek E S ES Houng H S HS Crawford R M RM Hadfield T L TL Hoover D L DL Warren R L RL
Infection and immunity 19950901 9
We constructed a defined purine-auxotrophic mutant of Brucella melitensis 16M by chromosomal gene replacement. We electroporated B. melitensis 16M with suicide plasmids containing a kanamycin resistance cassette that replaced 226 bp at the carboxyl end of purE, the intergenic region, and 18 bases of the purK open reading frame. Recombinant B. melitensis delta purE201 required exogenous purines for growth on minimal media. Purine auxotrophy was complemented by electroporation of B. melitensis del ...[more]