Unknown

Dataset Information

0

Inhibitory effect of oestradiol on activation of rat hepatic stellate cells in vivo and in vitro.


ABSTRACT: BACKGROUND: Hepatic stellate cells play a key role in the pathogenesis of hepatic fibrosis. AIMS: To examine the inhibitory effect of oestradiol on stellate cell activation. METHODS: In vivo, hepatic fibrosis was induced in rats by dimethylnitrosamine or pig serum. In vitro, rat stellate cells were activated by contact with plastic dishes resulting in their transformation into myofibroblast-like cells. RESULTS: In the dimethylnitrosamine and pig serum models, treatment with oestradiol at gestation related doses resulted in a dose dependent suppression of hepatic fibrosis with restored content of hepatic retinyl palmitate, reduced collagen content, lower areas of stellate cells which express alpha smooth muscle actin (alpha-SMA) and desmin, and lower procollagen type I and III mRNA levels in the liver. In cultured stellate cells, oestradiol inhibited type I collagen production, alpha-SMA expression, and cell proliferation. These findings suggest that oestradiol is a potent inhibitor of stellate cell transformation. CONCLUSION: The antifibrogenic role of oestradiol in the liver may contribute to the sex associated differences in the progression from hepatic fibrosis to cirrhosis

SUBMITTER: Shimizu I 

PROVIDER: S-EPMC1760074 | biostudies-other | 1999 Jan

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC4116159 | biostudies-literature
| S-EPMC2555962 | biostudies-literature
| S-EPMC5460523 | biostudies-literature
| S-EPMC3560665 | biostudies-literature
| S-EPMC3122263 | biostudies-literature
| S-EPMC7733620 | biostudies-literature
| S-EPMC5849734 | biostudies-literature
| S-EPMC3760729 | biostudies-literature
2023-02-07 | GSE223693 | GEO
| S-EPMC6378220 | biostudies-literature