Ontology highlight
ABSTRACT:
SUBMITTER: Nagy PL
PROVIDER: S-EPMC176736 | biostudies-other | 1995 Mar
REPOSITORIES: biostudies-other
Nagy P L PL Marolewski A A Benkovic S J SJ Zalkin H H
Journal of bacteriology 19950301 5
The enzyme encoded by Escherichia coli purU has been overproduced, purified, and characterized. The enzyme catalyzes the hydrolysis of 10-formyltetrahydrofolate (formyl-FH4) to FH4 and formate. Formyl-FH4 hydrolase thus generates the formate that is used by purT-encoded 5'-phosphoribosylglycinamide transformylase for step three of de novo purine nucleotide synthesis. Formyl-FH4 hydrolase, a hexamer with 32-kDa subunits, is activated by methionine and inhibited by glycine. Heterotropic cooperativ ...[more]