Unknown

Dataset Information

0

Cloning and characterization of the Bacillus subtilis birA gene encoding a repressor of the biotin operon.


ABSTRACT: The Bacillus subtilis birA gene, which regulates biotin biosynthesis, has been cloned and characterized. The birA gene maps at 202 degrees on the B. subtilis chromosome and encodes a 36,200-Da protein that is 27% identical to Escherichia coli BirA protein. Three independent mutations in birA that lead to deregulation of biotin synthesis alter single amino acids in the amino-terminal end of the protein. The amino-terminal region that is affected by these three birA mutations shows sequence similarity to the helix-turn-helix DNA binding motif previously identified in E. coli BirA protein. B. subtilis BirA protein also possesses biotin-protein ligase activity, as judged by its ability to complement a conditional lethal birA mutant of E. coli.

SUBMITTER: Bower S 

PROVIDER: S-EPMC176921 | biostudies-other | 1995 May

REPOSITORIES: biostudies-other

altmetric image

Publications

Cloning and characterization of the Bacillus subtilis birA gene encoding a repressor of the biotin operon.

Bower S S   Perkins J J   Yocum R R RR   Serror P P   Sorokin A A   Rahaim P P   Howitt C L CL   Prasad N N   Ehrlich S D SD   Pero J J  

Journal of bacteriology 19950501 9


The Bacillus subtilis birA gene, which regulates biotin biosynthesis, has been cloned and characterized. The birA gene maps at 202 degrees on the B. subtilis chromosome and encodes a 36,200-Da protein that is 27% identical to Escherichia coli BirA protein. Three independent mutations in birA that lead to deregulation of biotin synthesis alter single amino acids in the amino-terminal end of the protein. The amino-terminal region that is affected by these three birA mutations shows sequence simila  ...[more]

Similar Datasets

| S-EPMC178169 | biostudies-other
| S-EPMC532427 | biostudies-literature
| S-EPMC41358 | biostudies-other
| S-EPMC206107 | biostudies-other
| S-EPMC107026 | biostudies-literature
| S-EPMC101877 | biostudies-literature
| S-EPMC178230 | biostudies-other
| S-EPMC106171 | biostudies-literature
| S-EPMC106281 | biostudies-literature
| S-EPMC178458 | biostudies-literature