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Cloning and characterization of the Bacillus subtilis birA gene encoding a repressor of the biotin operon.

ABSTRACT: The Bacillus subtilis birA gene, which regulates biotin biosynthesis, has been cloned and characterized. The birA gene maps at 202 degrees on the B. subtilis chromosome and encodes a 36,200-Da protein that is 27% identical to Escherichia coli BirA protein. Three independent mutations in birA that lead to deregulation of biotin synthesis alter single amino acids in the amino-terminal end of the protein. The amino-terminal region that is affected by these three birA mutations shows sequence similarity to the helix-turn-helix DNA binding motif previously identified in E. coli BirA protein. B. subtilis BirA protein also possesses biotin-protein ligase activity, as judged by its ability to complement a conditional lethal birA mutant of E. coli.

SUBMITTER: Bower S

PROVIDER: S-EPMC176921 | biostudies-other | 1995 May

REPOSITORIES: biostudies-other

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Cloning and characterization of the Bacillus subtilis birA gene encoding a repressor of the biotin operon.

Bower S S Perkins J J Yocum R R RR Serror P P Sorokin A A Rahaim P P Howitt C L CL Prasad N N Ehrlich S D SD Pero J J

Journal of bacteriology 19950501 9

The Bacillus subtilis birA gene, which regulates biotin biosynthesis, has been cloned and characterized. The birA gene maps at 202 degrees on the B. subtilis chromosome and encodes a 36,200-Da protein that is 27% identical to Escherichia coli BirA protein. Three independent mutations in birA that lead to deregulation of biotin synthesis alter single amino acids in the amino-terminal end of the protein. The amino-terminal region that is affected by these three birA mutations shows sequence simila ...[more]

PMID: 7730294

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