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Cloning and primary structure of the chiA gene from Aeromonas caviae.


ABSTRACT: The chiA gene from Aeromonas caviae encodes an extracellular chitinase, 865 amino acids long, that shows a high degree of similarity to chitinase A of Serratia marcescens. Expression in Escherichia coli yielded an enzymatically active protein from which a leader sequence was removed, presumably during transport of the enzyme across the cell membrane.

SUBMITTER: Sitrit Y 

PROVIDER: S-EPMC177160 | biostudies-other | 1995 Jul

REPOSITORIES: biostudies-other

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Cloning and primary structure of the chiA gene from Aeromonas caviae.

Sitrit Y Y   Vorgias C E CE   Chet I I   Oppenheim A B AB  

Journal of bacteriology 19950701 14


The chiA gene from Aeromonas caviae encodes an extracellular chitinase, 865 amino acids long, that shows a high degree of similarity to chitinase A of Serratia marcescens. Expression in Escherichia coli yielded an enzymatically active protein from which a leader sequence was removed, presumably during transport of the enzyme across the cell membrane. ...[more]

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