Identification of Pseudomonas aeruginosa glpM, whose gene product is required for efficient alginate biosynthesis from various carbon sources.
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ABSTRACT:
SUBMITTER: Schweizer HP
PROVIDER: S-EPMC177247 | biostudies-other | 1995 Aug
REPOSITORIES: biostudies-other
Publications
Identification of Pseudomonas aeruginosa glpM, whose gene product is required for efficient alginate biosynthesis from various carbon sources.
Schweizer H P HP Po C C Bacic M K MK
Journal of bacteriology 19950801 16
In a mucB (algN) genetic background, insertion of an omega element approximately 200 bp downstream of glpD, encoding sn-glycerol-3-phosphate dehydrogenase from Pseudomonas aeruginosa, had an adverse effect on alginate biosynthesis from various carbon sources. The insertion inactivated glpM, a gene encoding a 12,040-M(r) hydrophobic protein containing 109 amino acids. This protein, which was expressed in a T7 RNA polymerase expression system, appears to be a cytoplasmic membrane protein. ...[more]
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