Isolation and sequencing of the replication region of Mycobacterium avium plasmid pLR7.
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ABSTRACT:
SUBMITTER: Beggs ML
PROVIDER: S-EPMC177255 | biostudies-other | 1995 Sep
REPOSITORIES: biostudies-other
Publications
Isolation and sequencing of the replication region of Mycobacterium avium plasmid pLR7.
Beggs M L ML Crawford J T JT Eisenach K D KD
Journal of bacteriology 19950901 17
The Mycobacterium avium plasmid pLR7 is representative of a group of small plasmids that are common in isolates from AIDS patients with disseminated M. avium infections. Determination of the functions of these and other plasmids has been hampered by the lack of methods for genetic manipulation of M. avium. In this study, the region of pLR7 capable of replication was identified and sequenced. Fragments of pLR7 were cloned into a pUC18 derivative carrying a kanamycin resistance marker and introduc ...[more]
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