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Molecular characterization and functional analysis of the major autolysin of Staphylococcus aureus 8325/4.

ABSTRACT: The gene encoding the major autolysin of Staphylococcus aureus 8325/4 has been cloned, sequenced, and insertionally inactivated. The three-domain, 137,384-Da protein has a C-terminal glucosaminidase active site and is involved in cell separation, generalized cell lysis, and release of wall material at the cell surface. Expression occurs throughout growth and is stimulated by low temperatures and in the presence of 1 M NaCl.

SUBMITTER: Foster SJ

PROVIDER: S-EPMC177389 | biostudies-other | 1995 Oct

REPOSITORIES: biostudies-other

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Molecular characterization and functional analysis of the major autolysin of Staphylococcus aureus 8325/4.

Foster S J SJ

Journal of bacteriology 19951001 19

The gene encoding the major autolysin of Staphylococcus aureus 8325/4 has been cloned, sequenced, and insertionally inactivated. The three-domain, 137,384-Da protein has a C-terminal glucosaminidase active site and is involved in cell separation, generalized cell lysis, and release of wall material at the cell surface. Expression occurs throughout growth and is stimulated by low temperatures and in the presence of 1 M NaCl. ...[more]

PMID: 7559367

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