Molecular analysis of the anaerobic succinate degradation pathway in Clostridium kluyveri.
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ABSTRACT:
SUBMITTER: Sohling B
PROVIDER: S-EPMC177737 | biostudies-other | 1996 Feb
REPOSITORIES: biostudies-other
Publications
Molecular analysis of the anaerobic succinate degradation pathway in Clostridium kluyveri.
Journal of bacteriology 19960201 3
A region of genomic DNA from Clostridium kluyveri was cloned in Escherichia coli by a screening strategy which was based on heterologous expression of the clostridial 4-hydroxybutyrate dehydrogenase gene. The gene region (6,575 bp) contained several open reading frames which encoded the coenzyme A (CoA)- and NADP+-dependent succinate-semialdehyde dehydrogenase (sucD), the 4-hydroxybutyrate dehydrogenase (4hbD), and a succinyl-CoA;CoA transferase (cat1), as analyzed by heterologous expression in ...[more]
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