Unknown

Dataset Information

0

Flagellin A is essential for the virulence of Vibrio anguillarum.


ABSTRACT: A flagellin gene from the fish pathogen Vibrio anguillarum was cloned, sequenced, and mutagenized. The DNA sequence suggests that the flaA gene encodes a 40.1-kDa protein and is a single transcriptional unit. A polar mutation and four in-frame deletion mutations (180 bp deleted from the 5' end of the gene, 153 bp deleted from the 3' end of the gene, a double deletion of both the 180- and 153-bp deletions, and 942 bp deleted from the entire gene) were made. Compared with the wild type, all mutants were partially motile, and a shortening of the flagellum was seen by electron microscopy. Wild-type phenotypes were regained when the mutations were transcomplemented with the flaA gene. Protein analysis indicated that the flaA gene corresponds to a 40-kDa protein and that the flagellum consists of three additional flagellin proteins with molecular masses of 41, 42, and 45 kDa. N-terminal sequence analysis confirmed that the additional proteins were flagellins with N termini that are 82 to 88% identical to the N terminus of FlaA. Virulence studies showed that the N terminal deletion, the double deletion, and the 942-bp deletion increased the 50% lethal dose between 70- and 700-fold via immersion infection, whereas infection via intraperitoneal injection showed no loss in virulence. In contrast, the polar mutant and the carboxy-terminal deletion mutant showed approximately a 10(4)-fold increase in the 50% lethal dose by both immersion and intraperitoneal infection. In summary, FlaA is needed for crossing the fish integument and may play a role in virulence after invasion of the host.

SUBMITTER: Milton DL 

PROVIDER: S-EPMC177804 | biostudies-other | 1996 Mar

REPOSITORIES: biostudies-other

altmetric image

Publications

Flagellin A is essential for the virulence of Vibrio anguillarum.

Milton D L DL   O'Toole R R   Horstedt P P   Wolf-Watz H H  

Journal of bacteriology 19960301 5


A flagellin gene from the fish pathogen Vibrio anguillarum was cloned, sequenced, and mutagenized. The DNA sequence suggests that the flaA gene encodes a 40.1-kDa protein and is a single transcriptional unit. A polar mutation and four in-frame deletion mutations (180 bp deleted from the 5' end of the gene, 153 bp deleted from the 3' end of the gene, a double deletion of both the 180- and 153-bp deletions, and 942 bp deleted from the entire gene) were made. Compared with the wild type, all mutant  ...[more]

Similar Datasets

| S-EPMC178316 | biostudies-other
| S-EPMC529167 | biostudies-literature
| S-EPMC8667730 | biostudies-literature
2024-09-02 | GSE184853 | GEO
| S-EPMC444792 | biostudies-literature
| S-EPMC207417 | biostudies-other
| S-EPMC8564382 | biostudies-literature
| S-EPMC3319172 | biostudies-literature
| S-EPMC193973 | biostudies-literature
| S-EPMC6083037 | biostudies-literature