Unknown

Dataset Information

0

A re-investigation of the path of carbon in photosynthesis utilizing GC/MS methodology. Unequivocal verification of the participation of octulose phosphates in the pathway.


ABSTRACT: A GC/EIMS/SIM methodology has been developed to re-examine the path of carbon in photosynthesis. Exposing isolated spinach chloroplasts to 13CO2 on a solid support for a defined period followed by quenching and work-up provided a mixture of labelled sugar phosphates. After enzymatic dephosphorylation and derivatization, the Mox-TMS sugars were analysed using the above method. The purpose of the study was to try to calculate the atom% enrichment of 13C in as many of the individual carbons in each of the derivatized sugars as was practical using diagnostic fragment ions. In the event, only one 45 s experiment provided sufficient data to enable a range of enrichment values to be calculated. This confirmed that D-glycero-D-altro-octulose phosphate was present in the chloroplasts and was heavily labelled in the C4, C5 and C6 positions, in keeping with the hypothesis that it had an inclusive role and a labelling pattern consistent with a new modified pathway of carbon in photosynthesis.

SUBMITTER: Flanigan IL 

PROVIDER: S-EPMC1779625 | biostudies-other | 2006 Nov

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC10742467 | biostudies-literature
| S-EPMC4849136 | biostudies-literature
| S-EPMC7254393 | biostudies-literature
| S-EPMC5847631 | biostudies-literature
| S-EPMC10458701 | biostudies-literature
| S-EPMC6920549 | biostudies-literature
| S-EPMC8231491 | biostudies-literature
| S-EPMC5533240 | biostudies-literature
| S-EPMC9562185 | biostudies-literature
| S-EPMC7033152 | biostudies-literature