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HrpXv, an AraC-type regulator, activates expression of five of the six loci in the hrp cluster of Xanthomonas campestris pv. vesicatoria.

ABSTRACT: hrp genes, basic pathogenicity genes of the pepper and tomato pathogen Xanthomonas campestris pv. vesicatoria, are regulated dependent on environmental conditions. We isolated the hrpXv gene, which was found to be outside the large hrp cluster comprising the six loci hrpA to hrpF. The predicted HrpXv protein is 476 amino acids long and has a molecular mass of 52.5 kDa. HrpX is highly conserved among xanthomonads and is a member of the AraC family of regulatory proteins. An hrpXv insertion mutant has a typical hrp phenotype and no longer allows induction of the five hrp loci hrpB to hrpF in the new hrp induction medium XVM2, indicating that HrpXv is the positive regulator of these loci. An hrpXv mutant could be partially complemented by the related hrpB gene of Burkholderia solanacearum, the protein product of which shows 40 and 58% amino acid identity and similarity, respectively, to HrpXv. The hrpXv gene itself has a low basal level of expression that is enhanced in XVM2. Expression of hrpXv as well as that of the hrpA locus is independent of the hrpXv gene. The transcription start site of hrpXv was mapped. Comparison between the hrpXv promoter and the corresponding region of the hrpXc gene from X. campestris pv. campestris revealed sequence conservation up to position -84. A putative helix-turn-helix motif in the C-terminal region of HrpXv and its possible interaction with a conserved hrp promoter element, the plant-inducible promoter box, are discussed.

SUBMITTER: Wengelnik K

PROVIDER: S-EPMC178114 | biostudies-other | 1996 Jun

REPOSITORIES: biostudies-other

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HrpXv, an AraC-type regulator, activates expression of five of the six loci in the hrp cluster of Xanthomonas campestris pv. vesicatoria.

Wengelnik K K Bonas U U

Journal of bacteriology 19960601 12

hrp genes, basic pathogenicity genes of the pepper and tomato pathogen Xanthomonas campestris pv. vesicatoria, are regulated dependent on environmental conditions. We isolated the hrpXv gene, which was found to be outside the large hrp cluster comprising the six loci hrpA to hrpF. The predicted HrpXv protein is 476 amino acids long and has a molecular mass of 52.5 kDa. HrpX is highly conserved among xanthomonads and is a member of the AraC family of regulatory proteins. An hrpXv insertion mutant ...[more]

PMID: 8655542

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Project description:We characterized the copper resistance genes in strain XvP26 of Xanthomonas campestris pv. vesicatoria, which was originally isolated from a pepper plant in Taiwan. The copper resistance genes were localized to a 7,652-bp region which, based on pulsed-field gel electrophoresis and Southern hybridization, was determined to be located on the chromosome. These genes hybridized only weakly, as determined by Southern analysis, to other copper resistance genes in Xanthomonas and Pseudomonas strains. We identified five open reading frames (ORFs) whose products exhibited high levels of amino acid sequence identity to the products of previously reported copper genes. Mutations in ORF1, ORF3, and ORF4 removed copper resistance, whereas mutations in ORF5 resulted in an intermediate copper resistance phenotype and insertions in ORF2 had no effect on resistance conferred to a copper-sensitive recipient in transconjugant tests. Based on sequence analysis, ORF1 was determined to have high levels of identity with the CopR (66%) and PcoR (63%) genes in Pseudomonas syringae pv. tomato and Escherichia coli, respectively. ORF2 and ORF5 had high levels of identity with the PcoS gene in E. coli and the gene encoding a putative copper-containing oxidoreductase signal peptide protein in Sinorhizobium meliloti, respectively. ORF3 and ORF4 exhibited 23% identity to the gene encoding a cation efflux system membrane protein, CzcC, and 62% identity to the gene encoding a putative copper-containing oxidoreductase protein, respectively. The latter two ORFs were determined to be induced following exposure to low concentrations of copper, while addition of Co, Cd, or Zn resulted in no significant induction. PCR analysis of 51 pepper and 34 tomato copper-resistant X. campestris pv. vesicatoria strains collected from several regions in Taiwan between 1987 and 2000 and nine copper-resistant strains from the United States and South America showed that successful amplification of DNA was obtained only for strain XvP26. The organization of this set of copper resistance genes appears to be uncommon, and the set appears to occur rarely in X. campestris pv. vesicatoria.

Dataset Information

HrpXv, an AraC-type regulator, activates expression of five of the six loci in the hrp cluster of Xanthomonas campestris pv. vesicatoria.

Publications

HrpXv, an AraC-type regulator, activates expression of five of the six loci in the hrp cluster of Xanthomonas campestris pv. vesicatoria.

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