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A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Escherichia coli.

ABSTRACT: A chromosomal DNA fragment from the erythromycin-sensitive bacterium Corynebacterium glutamicum ATCC 13032 was shown to mediate resistance against erythromycin, tetracycline, puromycin, and bleomycin in Escherichia coli. Multicopy cloning of the fragment did not cause a resistance phenotype in C. glutamicum. The corresponding gene encodes a hydrophobic protein with 12 potential transmembrane-spanning ex-helical segments showing similarity to drug-H+ antiporters.

SUBMITTER: Jager W

PROVIDER: S-EPMC178988 | biostudies-other | 1997 Apr

REPOSITORIES: biostudies-other

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A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Escherichia coli.

Jäger W W Kalinowski J J Pühler A A

Journal of bacteriology 19970401 7

A chromosomal DNA fragment from the erythromycin-sensitive bacterium Corynebacterium glutamicum ATCC 13032 was shown to mediate resistance against erythromycin, tetracycline, puromycin, and bleomycin in Escherichia coli. Multicopy cloning of the fragment did not cause a resistance phenotype in C. glutamicum. The corresponding gene encodes a hydrophobic protein with 12 potential transmembrane-spanning ex-helical segments showing similarity to drug-H+ antiporters. ...[more]

PMID: 9079937

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