A putative monofunctional glycosyltransferase is expressed in Ralstonia eutropha.
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ABSTRACT:
SUBMITTER: Paik J
PROVIDER: S-EPMC179221 | biostudies-other | 1997 Jun
REPOSITORIES: biostudies-other
Publications
A putative monofunctional glycosyltransferase is expressed in Ralstonia eutropha.
Paik J J Jendrossek D D Hakenbeck R R
Journal of bacteriology 19970601 12
A gene, mgt, encoding a protein homologous to the N-terminal module of class A high-molecular-mass penicillin-binding proteins was identified in Ralstonia eutropha. By using specific antibodies, the corresponding Mgt protein was detected in association with the membrane, confirming that the N-terminal hydrophobic segment functioned as a membrane anchor. A derivative in which the hydrophobic sequence was deleted was overexpressed as a maltose-binding fusion protein in Escherichia coli. Cleavage o ...[more]
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