Designation of Streptomycete 16S and 23S rRNA-based target regions for oligonucleotide probes.
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ABSTRACT:
SUBMITTER: Stackebrandt E
PROVIDER: S-EPMC182971 | biostudies-other | 1991 May
REPOSITORIES: biostudies-other
Publications
Designation of Streptomycete 16S and 23S rRNA-based target regions for oligonucleotide probes.
Stackebrandt E E Witt D D Kemmerling C C Kroppenstedt R R Liesack W W
Applied and environmental microbiology 19910501 5
The 16S and 23S rRNA of various Streptomyces species were partially sequenced and screened for the presence of stretches that could define all members of the genus, groups of species, or individual species. Nucleotide 929 (Streptomyces ambofaciens nomenclature [J.L. Pernodet, M.T. Alegre, F. Boccard, and M. Guerineau, Gene 79:33-46, 1989]) is a nucleotide highly unique to Streptomyces species which, in combination with flanking regions, allowed the designation of a genus-specific probe. Regions ...[more]
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