Characterization of the chromosomal aac(6')-Ii gene specific for Enterococcus faecium.
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ABSTRACT:
SUBMITTER: Costa Y
PROVIDER: S-EPMC188089 | biostudies-other | 1993 Sep
REPOSITORIES: biostudies-other
Publications
Characterization of the chromosomal aac(6')-Ii gene specific for Enterococcus faecium.
Costa Y Y Galimand M M Leclercq R R Duval J J Courvalin P P
Antimicrobial agents and chemotherapy 19930901 9
Chromosomal gene aac(6')-Ii of Enterococcus faecium CIP 54-32, encoding a 6'-N-aminoglycoside acetyltransferase was characterized. The gene was identified as a coding sequence of 549 bp corresponding to a protein with a calculated mass of 20,666 Da. Analysis of the sequence of the deduced protein suggested that it was the second member of a subfamily of AAC(6')-I enzymes. Insertional inactivation of aac(6')-Ii led to aminoglycoside susceptibility of CIP 54-32, suggesting that this gene plays a r ...[more]
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